Molecular cloning of human class IV alcohol dehydrogenase cDNA

Hirokazu Yokoyama, Enrique Baraona, Charles S. Lieber

研究成果: Article査読

28 被引用数 (Scopus)

抄録

A cDNA encoding a new type of alcohol dehydrogenase was cloned from a human stomach cDNA library. PCR amplification of 5’-stretch human stomach λgt11 library, using degenerate inosine-containing oligonucleotide probes compatible with peptide sequences of human σ-ADH, resulted in a single product. Subsequently, internal non-degenerate primers were constructed according to the sequences occurring in the product. By PCR with combinations its 5' and 3' ends were amplified. The full length cDNA sequence has 1125 nucleotides with a 72% similarity to those of human class I ADH. The polypeptide sequence, predicted from the cDNA, corresponds to 373 amino acids with a high degree of similarity (96%) to fragments of σ-ADH previously reported. Northern hybridization analysis with the specific probe for the mRNA of this protein showed that it is expressed in the human stomach but not in the liver. These data indicate that the cDNA we cloned is that of human class IV ADH.

本文言語English
ページ(範囲)219-224
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
203
1
DOI
出版ステータスPublished - 1994 1月 1
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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