Molecular cloning of rat macrophage-stimulating protein and its involvement in the male reproductive system

Kazuiku Ohshiro, Atsushi Iwama, Kenjiro Matsuno, Taichi Ezaki, Osamu Sakamoto, Isao Hamaguchi, Nobuyuki Takasu, Toshio Suda

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Macrophage-stimulating protein (MSP), a member of the hepatocyte growth factor family, is a ligand for receptor tyrosine kinase STK/RON. Here we isolated a full-length cDNA of rat MSP and a partial cDNA of rat STK/RON, then characterized their expression in the male reproductive system. In situ hybridization revealed that MSP mRNA was localized to spermatogonia and spermatocytes in the testis and the epithelium lining the lumen of the epididymis. On the other hand, RT-PCR analysis showed that STK/RON mRNA was expressed in sperm collected from both testis and epididymis. These findings suggest that locally produced MSP may play a vital role in germ cell-germ cell interaction during spermatogenesis as well as in the acquisition of sperm motility and/or fertilizing capacity in the epididymis. Our findings reveal new possible roles of the MSP-STK/RON signaling pathway.

本文言語English
ページ(範囲)273-280
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
227
1
DOI
出版ステータスPublished - 1996 10 3

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Molecular cloning of rat macrophage-stimulating protein and its involvement in the male reproductive system」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル