Molecular cloning of rat macrophage-stimulating protein and its involvement in the male reproductive system

Kazuiku Ohshiro, Atsushi Iwama, Kenjiro Matsuno, Taichi Ezaki, Osamu Sakamoto, Isao Hamaguchi, Nobuyuki Takasu, Toshio Suda

研究成果: Article

18 引用 (Scopus)

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Macrophage-stimulating protein (MSP), a member of the hepatocyte growth factor family, is a ligand for receptor tyrosine kinase STK/RON. Here we isolated a full-length cDNA of rat MSP and a partial cDNA of rat STK/RON, then characterized their expression in the male reproductive system. In situ hybridization revealed that MSP mRNA was localized to spermatogonia and spermatocytes in the testis and the epithelium lining the lumen of the epididymis. On the other hand, RT-PCR analysis showed that STK/RON mRNA was expressed in sperm collected from both testis and epididymis. These findings suggest that locally produced MSP may play a vital role in germ cell-germ cell interaction during spermatogenesis as well as in the acquisition of sperm motility and/or fertilizing capacity in the epididymis. Our findings reveal new possible roles of the MSP-STK/RON signaling pathway.

元の言語English
ページ(範囲)273-280
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
227
発行部数1
DOI
出版物ステータスPublished - 1996 10 3

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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