Molecular design of near-infrared (NIR) fluorescent probes targeting exopeptidase and application for detection of dipeptidyl peptidase 4 (DPP-4) activity

Yuki Hoshino, Kenjiro Hanaoka, Kei Sakamoto, Masahiro Yasunaga, Takashi Kojima, Daisuke Kotani, Ayumu Nomoto, Eita Sasaki, Toru Komatsu, Tasuku Ueno, Hiroyuki Takamaru, Yutaka Saito, Yasuyuki Seto, Yasuteru Urano

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Monitoring the activities of proteases in vivo is an important requirement in biological and medical research. Near-infrared (NIR) fluorescent probes are particularly useful for in vivo fluorescence imaging, due to the high penetration of NIR and the low autofluorescence in tissue for this wavelength region, but most current NIR fluorescent probes for proteases are targeted to endopeptidase. Here, we describe a new molecular design for NIR fluorescent probes that target exopeptidase by utilizing the >110 nm blueshift of unsymmetrical Si-rhodamines upon amidation of the N atom of their xanthene moiety. Based on this molecular design, we developed Leu-SiR640 as a probe for leucine amino peptidase (LAP). Leu-SiR640 shows a one order of magnitude larger fluorescence increment (669-fold) upon reaction with LAP than existing NIR fluorescent probes. We similarly designed and synthesized EP-SiR640, a NIR fluorescent probe that targets dipeptidyl peptidase 4 (DPP-4). We show that this probe can monitor DPP-4 activity not only in living cells but also in mouse organs and tumors. This probe could also detect esophageal cancer in human clinical specimens, based on the overexpression of DPP-4 activity.

本文言語English
ページ(範囲)859-867
ページ数9
ジャーナルRSC Chemical Biology
3
7
DOI
出版ステータスPublished - 2022 3月 24

ASJC Scopus subject areas

  • 化学(その他)
  • 生化学
  • 分子生物学
  • 生化学、遺伝学、分子生物学(その他)

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