Molecular Genetic Analysis of Myelin‐Deficient Mice: Shiverer Mutant Mice Show Deletion in Gene(s) Coding for Myelin Basic Protein

Minoru Kimura, Hidetoshi Inoko, Motoya Katsuki, Asako Ando, Tadayuki Sato, Tadaaki Hirose, Hideaki Takashima, Seiichi Inayama, Hideyuki Okano, Ken Takamatsu, Katsuhiko Mikoshiba, Yasuzo Tsukada, Itaru Watanabe

研究成果: Article査読

70 被引用数 (Scopus)

抄録

Abstract: The gene expression of myelin basic proteins (MBPs) in shiverer mutant mice was investigated by the Northern and Southern hybridization techniques. In the control mice RNA molecules from the brains which were about 2,300 nucleotides in length were hybridized to cDNA of 1.8 kb encoding for a mouse MBP, but RNA from the brains of 3‐week‐old shiverer mutant mice contained no detectable amount of MBP transcripts hybridizing to this probe. Moreover the shiverer mutant mice lost several restriction fragments that hybridized to the same probe in the control mice when each of the five restriction enzymes, i.e., HindIII, PstI, PvuII, AccI, and StuI, was used. These data suggest that the shiverer mutation may correspond to the deletion of a large portion of MBP exon(s) in the gene, and this deletion causes inefficient transcription leading to the depletion of MBPs in the myelin and the dysmyelination observed in these mice.

本文言語English
ページ(範囲)692-696
ページ数5
ジャーナルJournal of Neurochemistry
44
3
DOI
出版ステータスPublished - 1985 3

ASJC Scopus subject areas

  • 生化学
  • 細胞および分子神経科学

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