Molecular mass measurement of polymerase chain reaction products amplified from human blood dna by electrospray ionization mass spectrometry

Yasuhiro Naito, Keiichiro Ishikawa, Yoshinori Koga, Toshihiro Tsuneyoshi, Hideya Terunuma, Ryuichi Arakawa

研究成果: Article査読

54 被引用数 (Scopus)

抄録

We report here on the first analysis of polymerase chain reaction (PCR) products amplified from a small amount of human blood DNA by electrospray ionization mass spectrometry (ESI‐MS). Adenomatous polyposis coli (APC) gene fragment of about 50 base pairs (bp) with a relative molecular mass (Mr) of approximately 15 000 u was amplified from human blood DNA by PCR. The accurate molecular mass of the PCR products was determined with an accuracy of ∼0.005% by ESI‐MS. The amount of DNA used was only 100 ng (∼ 50 zmol; the theoretically required amount of blood is therefore less than 1 μL for PCR). The ESI‐MS measurement of the PCR products proved to be a new accurate, sensitive and fast tool for gene diagnosis.

本文言語English
ページ(範囲)1484-1486
ページ数3
ジャーナルRapid Communications in Mass Spectrometry
9
15
DOI
出版ステータスPublished - 1995
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 分光学
  • 有機化学

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