Molecular mechanisms of lipopolysaccharide-induced cyclooxygenase-2 expression in human neutrophils: Involvement of the mitogen-activated protein kinase pathway and regulation by anti-inflammatory cytokines

Shuji Nagano, Takeshi Otsuka, Hiroaki Niiro, Kunihiro Yamaoka, Yojirou Arinobu, Eiichi Ogami, Mitsuteru Akahoshi, Yasushi Inoue, Katsuhisa Miyake, Hitoshi Nakashima, Yoshiyuki Niho, Mine Harada

研究成果: Article査読

51 被引用数 (Scopus)

抄録

Neutrophils are an important cellular source of proinflammatory mediators, whose regulation may be of potential benefit for the treatment of a number of inflammatory diseases. However, the mechanisms of lipopolysaccharide (LPS)-induced neutrophil activation and its regulation by anti-inflammatory cytokines have not yet been fully elucidated. Recent studies have revealed that mitogen-activated protein kinases (MAPK) play a crucial role in the generation of proinflammatory mediators in some cell types. Therefore, we conducted this study to determine whether MAPK activation could be involved in prostaglandin E2 (PGE2) production and cyclooxygenase (COX)-2 expression in LPS-stimulated human neutrophils. PD98059 (MEK1 inhibitor) and SB203580 (p38MAPK inhibitor) reduced PGE2 production as well as COX-2 expression in LPS-stimulated neutrophils. In addition, both extracellular signal-regulated protein kinase (ERK) and p38MAPK were phosphorylated and activated in time- and dose-dependent manners. Since we previously showed that IL-10 and IL-4 similarly inhibited COX-2 expression in LPS-stimulated neutrophils, we next tested the effects of IL-10 and IL-4 on the phosphorylation and activation of both kinases. IL-10 inhibited the phosphorylation and activation of p38MAPK, but not ERK. In addition, IL-4 caused a marginal inhibition in the activation of p38MAPK. Taken together, these results suggest that both ERK and p38MAPK pathways are involved in LPS-induced COX-2 expression and PGE2 production in neutrophils, and IL-10 and IL-4 inhibit neutrophil prostanoid synthesis by down-regulating the activation of p38MAPK.

本文言語English
ページ(範囲)733-740
ページ数8
ジャーナルInternational immunology
14
7
DOI
出版ステータスPublished - 2002

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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