Molecular species analysis of glycosphingolipids from small intestine of Japanese quail, Coturnix coturnix Japonica by HPLC/FAB/MS

Atsushi C. Suzuki, Akira Nakamura, Kenji Nishimura

研究成果: Article査読

抄録

Neutral glycosphingolipids were isolated from quail small intestine and their structures were analysed. They contained: Galβ1-4GlcCer(LacCer), Galα1-4GalCer(Ga2Cer), Galα1-4Galβ1-4GlcCer(Gb3Cer), GlcNAcβ1-3Galβ1-4GlcCer(Le3Cer), GalNAcβ1-4Galβ1-4GlcCer(Gg3Cer), GalNAcβ1-4[GalNAcβ1-3]Galβ1-4GlcCer(LcGg4Cer), and GalNAcα1-3GalNAcβ1-3Galα1-4Galβ1-4GlcCer (Forssman glycolipid) as well as glucosylceramide, galactosylceramide (Nishimura K et al. 1984)Biochim Biophys Acta796:269-76) and the Lex glycolipid, III3 Fucα-nLc4Cer (Nishimura K et al. (1989)J. Biochem (Tokyo)101:1315-18). The molecular species compositions of these glycosphingolipids were examined using fast atom bombardment-mass spectrometry linked with reversed-phase high-performance liquid chromatography. By such analysis, we could classify the quail glycosphingolipids into at least three classes: glycolipids rich in species having four hydroxyl groups in the ceramides (GalCer, Gg3Cer, LcGg4Cer and Lex), those rich in the ceramides of N-acyl trihydroxysphinganine with normal fatty acids (Lc3Cer), and glycolipids rich in the ceramides of N-acyl sphingenine with normal fatty acids (LacCer, Gb3Cer and Forssman glycolipid). Immunohistochemical observation implies that the differences in the hydrophobic moieties specified the localization of glycosphingolipids in the tissue.

本文言語English
ページ(範囲)111-121
ページ数11
ジャーナルGlycoconjugate Journal
11
2
DOI
出版ステータスPublished - 1994 4月 1

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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