Molybdophyllysin, a toxic metalloendopeptidase from the tropical toadstool, Chlorophyllum molybdites

Mina Yamada, Naoko Tokumitsu, Yoko Saikawa, Masaya Nakata, Junpei Asano, Kazuo Miyairi, Toshikatsu Okuno, Katsuhiro Konno, Kimiko Hashimoto

研究成果: Article

3 引用 (Scopus)

抄録

A toxic protein, dubbed molybdophyllysin, was isolated from the tropical toadstool Chlorophyllum molybdites by following its lethal effect in mice. Analysis of the protein using SDS-PAGE revealed a single 23-kDa band. Sequence analysis of molybdophyllysin tryptic fragments showed that this protein is highly homologous to metalloendopeptidases (MEPs) obtained from edible mushrooms, such as Grifola frondosa, Pleurotus ostreatus, and Armillaria mellea. These proteins include a HEXXH+D zinc-binding motif known as aspzincin. Accordingly, molybdophyllysin is a member of the deuterolysin family of zinc proteases. Molybdophyllysin retained its proteolytic activity at temperatures up to 60 °C with an optimum pH of 7.0. The activity was inhibited by both 1,10-phenanthroline and N-bromosuccinimide, but molybdophyllysin exhibited strong resistance to SDS.

元の言語English
ページ(範囲)6583-6588
ページ数6
ジャーナルBioorganic and Medicinal Chemistry
20
発行部数22
DOI
出版物ステータスPublished - 2012 11 15

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Metalloendopeptidases
Poisons
Zinc
Armillaria
Proteins
Grifola
Bromosuccinimide
Pleurotus
Agaricales
Sequence Analysis
Polyacrylamide Gel Electrophoresis
Peptide Hydrolases
Temperature

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry
  • Biochemistry

これを引用

Molybdophyllysin, a toxic metalloendopeptidase from the tropical toadstool, Chlorophyllum molybdites. / Yamada, Mina; Tokumitsu, Naoko; Saikawa, Yoko; Nakata, Masaya; Asano, Junpei; Miyairi, Kazuo; Okuno, Toshikatsu; Konno, Katsuhiro; Hashimoto, Kimiko.

:: Bioorganic and Medicinal Chemistry, 巻 20, 番号 22, 15.11.2012, p. 6583-6588.

研究成果: Article

Yamada, M, Tokumitsu, N, Saikawa, Y, Nakata, M, Asano, J, Miyairi, K, Okuno, T, Konno, K & Hashimoto, K 2012, 'Molybdophyllysin, a toxic metalloendopeptidase from the tropical toadstool, Chlorophyllum molybdites', Bioorganic and Medicinal Chemistry, 巻. 20, 番号 22, pp. 6583-6588. https://doi.org/10.1016/j.bmc.2012.09.036
Yamada, Mina ; Tokumitsu, Naoko ; Saikawa, Yoko ; Nakata, Masaya ; Asano, Junpei ; Miyairi, Kazuo ; Okuno, Toshikatsu ; Konno, Katsuhiro ; Hashimoto, Kimiko. / Molybdophyllysin, a toxic metalloendopeptidase from the tropical toadstool, Chlorophyllum molybdites. :: Bioorganic and Medicinal Chemistry. 2012 ; 巻 20, 番号 22. pp. 6583-6588.
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abstract = "A toxic protein, dubbed molybdophyllysin, was isolated from the tropical toadstool Chlorophyllum molybdites by following its lethal effect in mice. Analysis of the protein using SDS-PAGE revealed a single 23-kDa band. Sequence analysis of molybdophyllysin tryptic fragments showed that this protein is highly homologous to metalloendopeptidases (MEPs) obtained from edible mushrooms, such as Grifola frondosa, Pleurotus ostreatus, and Armillaria mellea. These proteins include a HEXXH+D zinc-binding motif known as aspzincin. Accordingly, molybdophyllysin is a member of the deuterolysin family of zinc proteases. Molybdophyllysin retained its proteolytic activity at temperatures up to 60 °C with an optimum pH of 7.0. The activity was inhibited by both 1,10-phenanthroline and N-bromosuccinimide, but molybdophyllysin exhibited strong resistance to SDS.",
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AU - Saikawa, Yoko

AU - Nakata, Masaya

AU - Asano, Junpei

AU - Miyairi, Kazuo

AU - Okuno, Toshikatsu

AU - Konno, Katsuhiro

AU - Hashimoto, Kimiko

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