Monitoring of WT1-specific cytotoxic T lymphocytes after allogeneic hematopoietic stem cell transplantation

Yuriko Morita, Yuji Heike, Mami Kawakami, Osamu Miura, Shin Ichi Nakatsuka, Michiko Ebisawa, Shin Ichiro Mori, Ryuji Tanosaki, Takahiro Fukuda, Sung Won Kim, Kensei Tobinai, Yoichi Takaue

研究成果: Article査読

36 被引用数 (Scopus)

抄録

Donor-derived cytotoxic T lymphocytes (CTL) that respond to tumor antigens emerge after hematopoietic stem cell transplantation (HSCT), particularly in association with the status of immune recovery. To analyze the frequency of CTL against PR1, PRAME and WT1 after HSCT, a tetramer-based analysis was performed in 97 samples taken from 35 patients (9 AML, 11 MDS, 2 CML, 4 ALL, 7 lymphoma and 2 renal cell carcinoma [RCC]) with the HLA-A02 phenotype. Regarding PR1, only 1 sample showed the presence of tetramer-positive cells (0.04%/lymphocyte). Similarly, in PRAME, only 10 of 97 samples were sporadically positive with low titers. For WT1, positive results were detected in 39 of 97 samples and 7 (2 CML, 1 ALL, 2 lymphoma and 2 RCC) patients clearly showed positive results more than once. On the basis of these results, we performed serial analyses of WT1-specific CTL during the clinical course in 2 patients with RCC, who underwent HSCT with a reduced-intensity regimen, to examine the precise correlation between the kinetics of CTL, the occurrence of GVHD and the observed clinical response. A higher positive rate for WT1-speciflc CTL and a correlation with the clinical response suggest that WT1 may be a useful antigen for a wider monitoring application.

本文言語English
ページ(範囲)1360-1367
ページ数8
ジャーナルInternational Journal of Cancer
119
6
DOI
出版ステータスPublished - 2006 9 15
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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