Mouse ovary developmental RNA and protein markers from gene expression profiling

Luisa Herrera, Chris Ottolenghi, J. Elias Garcia-Ortiz, Massimo Pellegrini, Francesca Manini, Minoru S.H. Ko, Ramaiah Nagaraja, Antonino Forabosco, David Schlessinger

研究成果: Article査読

52 被引用数 (Scopus)

抄録

To identify genes involved in morphogenetic events during mouse ovary development, we started with microarray analyses of whole organ RNA. Transcripts for 60% of the 15,000 gene NIA panel were detected, and about 2000 were differentially expressed in nascent newborn compared to adult ovary. Highly differentially expressed transcripts included noncoding RNAs and newly detected genes involved in transcription regulation and signal transduction. The phased pattern of newborn mouse ovary differentiation allowed us to (1) extend information on activity and stage specificity of cell type-specific genes; and (2) generate a list of candidate genes involved in primordial follicle formation, including podocalyxin (Podxl), PDGFR-β, and a follistatin-domain-encoding gene Flst1. Oocyte-specific transcripts included many (e.g., Deltex2, Bicd2, and Zfp37) enriched in growing oocytes, as well as a novel family of untranslated RNA's (RLTR10) that is selectively expressed in early stage follicles. The results indicate that global expression profiling of whole organ RNA provides sensitive first-line information about ovarian histogenesis for which no in vitro cell models are currently available.

本文言語English
ページ(範囲)271-290
ページ数20
ジャーナルDevelopmental Biology
279
2
DOI
出版ステータスPublished - 2005 3月 15
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 発生生物学
  • 細胞生物学

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