MRI Characterization of Paranodal Junction Failure and Related Spinal Cord Changes in Mice

Morito Takano, Keigo Hikishima, Kanehiro Fujiyoshi, Shinsuke Shibata, Akimasa Yasuda, Tsunehiko Konomi, Akiko Hayashi, Hiroko Baba, Koichi Honke, Yoshiaki Toyama, Hideyuki Okano, Masaya Nakamura

研究成果: Article査読

15 被引用数 (Scopus)

抄録

The paranodal junction is a specialized axon-glia contact zone that is important for normal neuronal activity and behavioral locomotor function in the central nervous system (CNS). Histological examination has been the only method for detecting pathological paranodal junction conditions. Recently, diffusion tensor MRI (DTI) has been used to detect microstructural changes in various CNS diseases. This study was conducted to determine whether MRI and DTI could detect structural changes in the paranodal junctions of the spinal cord in cerebroside sulfotransferase knock-out (CST-KO) mice. Here, we showed that high-resolution MRI and DTI characteristics can reflect paranodal junction failure in CST-KO mice. We found significantly lower T1 times and significantly higher T2 times in the spinal cord MRIs of CST-KO mice as compared to wild-type (WT) mice. Spinal cord DTI showed significantly lower axial diffusivity and significantly higher radial diffusivity in CST-KO mice as compared to WT mice. In contrast, the histological differences in the paranodal junctions of WT and CST-KO mice were so subtle that electron microscopy or immunohistological analyses were necessary to detect them. We also measured gait disturbance in the CST-KO mice, and determined the conduction latency by electrophysiology. These findings demonstrate the potential of using MRI and DTI to evaluate white matter disorders that involve paranodal junction failure.

本文言語English
論文番号e52904
ジャーナルPloS one
7
12
DOI
出版ステータスPublished - 2012 12 27

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 一般

フィンガープリント

「MRI Characterization of Paranodal Junction Failure and Related Spinal Cord Changes in Mice」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル