In order to correlate the yield and quality of shiitake mushrooms with the growth process of shiitake mycelium in logs over several years, a non-invasive visualization method using a magnetic resonance imaging (MRI) measurement system was developed. The MRI measurement system had a permanent magnet of 1.0 T magnetic field strength and a 110 mm air gap. The test logs in which grew the shiitake mycelium had a diameter of 43–48 mm, a length of 110–118 mm and a dried mass of 125–135 g. The log was inoculated on one cutting surface after being supplied water, and was placed in a thermostatic chamber (21 °C, about 75% humidity) for 69 days. The test log was observed by MRI, and MR images and T1 maps of shiitake mycelium growing over time were obtained. It was found that the shiitake mycelium has a T1 relaxation time constant longer than 150–200 ms, and its signal intensity in the MR images is smaller than that of water penetrated into a log. When making the MRI measurements, it is necessary to dry a log to water content of about 20% or less. In addition, a control log with mold was also observed by MRI. The shiitake mycelium can be distinguished from water penetrated into log and mold occurring in the log by combining the T1—signal intensity map and clear recognition of a low-intensity region in MR images acquired at longer echo times.
ASJC Scopus subject areas
- Polymers and Plastics