Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk

Nobuaki Kono, Hiroyuki Nakamura, Masaru Mori, Yuki Yoshida, Rintaro Ohtoshi, Ali D. Malay, Daniel A. Pedrazzoli Moran, Masaru Tomita, Keiji Numata, Kazuharu Arakawa

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Dragline silk of golden orb-weaver spiders (Nephilinae) is noted for its unsurpassed toughness, combining extraordinary extensibility and tensile strength, suggesting industrial application as a sustainable biopolymer material. To pinpoint the molecular composition of dragline silk and the roles of its constituents in achieving its mechanical properties, we report a multiomics approach, combining highquality genome sequencing and assembly, silk gland transcriptomics, and dragline silk proteomics of four Nephilinae spiders.We observed the consistent presence of the MaSp3B spidroin unique to this subfamily as well as several nonspidroin SpiCE proteins. Artificial synthesis and the combination of these components in vitro showed that the multicomponent nature of dragline silk, including MaSp3B and SpiCE, along with MaSp1 and MaSp2, is essential to realize the mechanical properties of spider dragline silk.

本文言語English
論文番号e2107065118
ジャーナルProceedings of the National Academy of Sciences of the United States of America
118
31
DOI
出版ステータスPublished - 2021 8 3

ASJC Scopus subject areas

  • 一般

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