TY - JOUR
T1 - Multiple mRNA species of choline acetyltransferase from rat spinal cord
AU - Kengaku, Mineko
AU - Misawa, Hidemi
AU - Deguchi, Takeo
N1 - Funding Information:
Acknowledgments. We thank Professor Seiichiro Kawashima, Zoological Institute, Faculty of Science, University of Tokyo, and Dr. Ryosuke Takahashi, Department of Neurology in this Institute for their advice and discussions, and Mrs. Michiko Tsuchikura for her secretarial assistance. This work was supported in part by Grants-in-Aid for Encouragement of Young Scientists to H.M. and for Scientific Research on Priority Areas (Molecular Basis of Neural Connections) to T.D. from the Ministry of Education, Science and Culture, and by Special Coordination Funds of the Science and Technology Agency of Japanese Government.
PY - 1993/4
Y1 - 1993/4
N2 - A cDNA library directed by a specific primer was constructed from the rat spinal cord and screened with 32P-labeled rat choline acetyltransferase cDNA which was recently isolated in this laboratory. Sequence analysis of 29 clones indicated that there are four types of cDNA (R1-, R2-, N1- and M-types). The nucleotide sequences in these cDNAs were identical in the coding region and the first 38 bp of the 5′-noncoding region, but differed in the 5′-noncoding region upstream of -38 bp. The R1-type was identical to the cDNA previously cloned from the rat spinal cord. The M and N1-type cDNAs both had sequences homologous to that of the cDNA previously obtained from the mouse spinal cord. Polymerase chain reaction analysis confirmed the presence of these 4 types of mRNA and found another type (N2-type) of transcript. The numbers of cDNA clones isolated and the relative amounts of polymerase chain reaction products for each type of mRNA suggested that the most abundant transcript was M-type. Sequencing of the genomic clone containing the 5′-region of choline acetyltransferase mRNA revealed that these five types of mRNA species were transcribed from three different promoter regions and produced by differential splicing of the 5′-noncoding exons.
AB - A cDNA library directed by a specific primer was constructed from the rat spinal cord and screened with 32P-labeled rat choline acetyltransferase cDNA which was recently isolated in this laboratory. Sequence analysis of 29 clones indicated that there are four types of cDNA (R1-, R2-, N1- and M-types). The nucleotide sequences in these cDNAs were identical in the coding region and the first 38 bp of the 5′-noncoding region, but differed in the 5′-noncoding region upstream of -38 bp. The R1-type was identical to the cDNA previously cloned from the rat spinal cord. The M and N1-type cDNAs both had sequences homologous to that of the cDNA previously obtained from the mouse spinal cord. Polymerase chain reaction analysis confirmed the presence of these 4 types of mRNA and found another type (N2-type) of transcript. The numbers of cDNA clones isolated and the relative amounts of polymerase chain reaction products for each type of mRNA suggested that the most abundant transcript was M-type. Sequencing of the genomic clone containing the 5′-region of choline acetyltransferase mRNA revealed that these five types of mRNA species were transcribed from three different promoter regions and produced by differential splicing of the 5′-noncoding exons.
KW - Alternative splicing
KW - Choline acetyltransferase
KW - Gene expression
KW - Genomic DNA
KW - Rat
KW - Spinal cord
KW - cDNA cloning
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U2 - 10.1016/0169-328X(93)90174-N
DO - 10.1016/0169-328X(93)90174-N
M3 - Article
C2 - 8479291
AN - SCOPUS:0027466842
VL - 18
SP - 71
EP - 76
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1-2
ER -