Multipotent and Committed CD34+ Cells in Bone Marrow Transplantation

Hideo Ema, Toshio Suda, Hiromitsu Nakauchi, Yukio Nakamura, Atsushi Iwama, Shigehiko Imagawa, Miyuki Akutsu, Yasuhiko Kano, Shunichi Kato, Miharu Yabe, Minoru Yoshida, Shinobu Sakamoto, Youichi Amemiya, Yasusada Miura

研究成果: Article

4 被引用数 (Scopus)

抄録

In order to study the role of CD34+ cells in hematological recovery following bone marrow transplantation (BMT), bone marrow cells stained with HPCA‐1 (CD34) and MY‐9 (CD33) monoclonal antibodies were analyzed by using a fluorescence‐activated cell sorter on or about days 14 and 28, as well as at later times, following BMT in 6 recipients. Single cell cultures of CD34+ cells were also performed to evaluate their in vitro hematopoietic function. CD34+ cells were detectable in bone marrow cells on day 14. More than 80% of CD34+ cells co‐expressed the CD33 antigen, and macrophage (Mac) colony‐forming cells predominated among total colony‐forming cells of CD34+ cells. In normal bone marrow cells, CD34+, CD33+ cells amounted to about 40% of CD34+ cells, and the incidences of erythroid bursts, granulocyte/macrophage (GM) colonies, and Mac colonies were similar to each other. After more than 10 weeks, CD34+, CD33 cells gradually recovered, as erythroid burst colony‐forming cells increased following GM colony‐forming cells. This phenomenon was well‐correlated with the time course of peripheral blood cell recovery. CD34+, CD33+ cells as committed progenitors and CD34+, CD33 cells as multipotent stem cells have distinctive biological behaviors in BMT.

本文言語English
ページ(範囲)547-552
ページ数6
ジャーナルJapanese Journal of Cancer Research
82
5
DOI
出版ステータスPublished - 1991 5

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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