Mutant screening for oncogenes of Ewing’s sarcoma using yeast

Takao Kitagawa, Hajime Okita, Byron Baron, Kazuhiro Tokuda, Mikiko Nakamura, Yufeng Wang, Junko Akada, Hisashi Hoshida, Rinji Akada, Yasuhiro Kuramitsu, Kazuyuki Nakamura

研究成果: Article査読

抄録

Many fusion genes, which are the result of chromosomal translocation and work as an oncogene, have been recently identified, but their mode of actions is still unclear. Here, we performed a yeast mutant screening for oncogenes of Ewing’s sarcoma to easily identify essential regions responsible for fusion protein functions using a yeast genetic system. Three kinds of oncogenes including EWS/FLI1, EWS/ERG, and EWS/E1AF exhibited growth inhibition in yeast. In this screening, we identified 13 single amino acid substitution mutants which could suppress growth inhibition by oncogenes. All of the point mutation positions of the EWS/ETS family proteins were located within the ETS domain, which is responsible for the interaction with a specific DNA motif. Eight-mutated residues within the ETS domain matched to 13 completely conserved amino acid residues in the human ETS domains. Moreover, mutants also showed reduced transcriptional activities on the DKK2 promoter, which is upregulated by the EWS/ETS family, compared to that of the wild type. These results suggest that the ETS domain in the EWS/ETS family proteins may be a primary target for growth inhibition of Ewing’s sarcoma and that this yeast screening system can be applied for the functional screening of the oncogenes.

本文言語English
ページ(範囲)6737-6744
ページ数8
ジャーナルApplied Microbiology and Biotechnology
99
16
DOI
出版ステータスPublished - 2015 8月 27

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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