TY - JOUR
T1 - Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model
AU - Osaki, Takako
AU - Hanawa, Tomoko
AU - Manzoku, Taki
AU - Fukuda, Minoru
AU - Kawakami, Hayato
AU - Suzuki, Hidekazu
AU - Yamaguchi, Hiroyuki
AU - Yan, Xu
AU - Taguchi, Haruhiko
AU - Kurata, Satoshi
AU - Kamiya, Shigeru
PY - 2006/11
Y1 - 2006/11
N2 - Helicobacter pylori is associated with gastric disorders in humans and some experimental animals, and possesses the luxS/type 2 autoinducer (Al-2) system. The effects of a specific luxS mutation on the characteristics of H. pylori were examined. On 0·3% agar medium, motility of H. pylori HPKY08 (luxS::cat) was significantly lower than that of wild-type H. pylori TK1402, The luxS-complemented strain HPKY21 exhibited motility comparable to that of H. pylori TK1402. It was shown that the luxS/Al-2 system plays an important role in H. pylori motility. The luxS mutant exhibited a reduced infection rate relative to the wild-type parent strain TK1402 in a Mongolian gerbil model. At 3 months after oral inoculation, lower numbers of H. pylori were detected by semi-quantitative real-time reverse transcription PCR (qRT-PCR) in luxS - mutant-infected gerbils than in TK1402-infected gerbils. Gastric inflammation and increased antibody titre for H. pylori were observed in TK1402-infected gerbils only.
AB - Helicobacter pylori is associated with gastric disorders in humans and some experimental animals, and possesses the luxS/type 2 autoinducer (Al-2) system. The effects of a specific luxS mutation on the characteristics of H. pylori were examined. On 0·3% agar medium, motility of H. pylori HPKY08 (luxS::cat) was significantly lower than that of wild-type H. pylori TK1402, The luxS-complemented strain HPKY21 exhibited motility comparable to that of H. pylori TK1402. It was shown that the luxS/Al-2 system plays an important role in H. pylori motility. The luxS mutant exhibited a reduced infection rate relative to the wild-type parent strain TK1402 in a Mongolian gerbil model. At 3 months after oral inoculation, lower numbers of H. pylori were detected by semi-quantitative real-time reverse transcription PCR (qRT-PCR) in luxS - mutant-infected gerbils than in TK1402-infected gerbils. Gastric inflammation and increased antibody titre for H. pylori were observed in TK1402-infected gerbils only.
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U2 - 10.1099/jmm.0.46660-0
DO - 10.1099/jmm.0.46660-0
M3 - Article
C2 - 17030905
AN - SCOPUS:33750838005
SN - 0022-2615
VL - 55
SP - 1477
EP - 1485
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
IS - 11
ER -