Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model

Takako Osaki, Tomoko Hanawa, Taki Manzoku, Minoru Fukuda, Hayato Kawakami, Hidekazu Suzuki, Hiroyuki Yamaguchi, Xu Yan, Haruhiko Taguchi, Satoshi Kurata, Shigeru Kamiya

研究成果: Article査読

44 被引用数 (Scopus)

抄録

Helicobacter pylori is associated with gastric disorders in humans and some experimental animals, and possesses the luxS/type 2 autoinducer (Al-2) system. The effects of a specific luxS mutation on the characteristics of H. pylori were examined. On 0·3% agar medium, motility of H. pylori HPKY08 (luxS::cat) was significantly lower than that of wild-type H. pylori TK1402, The luxS-complemented strain HPKY21 exhibited motility comparable to that of H. pylori TK1402. It was shown that the luxS/Al-2 system plays an important role in H. pylori motility. The luxS mutant exhibited a reduced infection rate relative to the wild-type parent strain TK1402 in a Mongolian gerbil model. At 3 months after oral inoculation, lower numbers of H. pylori were detected by semi-quantitative real-time reverse transcription PCR (qRT-PCR) in luxS - mutant-infected gerbils than in TK1402-infected gerbils. Gastric inflammation and increased antibody titre for H. pylori were observed in TK1402-infected gerbils only.

本文言語English
ページ(範囲)1477-1485
ページ数9
ジャーナルJournal of Medical Microbiology
55
11
DOI
出版ステータスPublished - 2006 11

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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