Myeloid and erythroid lineage expression of haemopoietic progenitors derived from an abnormal clone in erythroleukaemia

Toshio Suda, Yuko Sato, Yusuke Furukawa, Junko Suda, Mituoki Eguchi, Masaki Saito, Yasusada Miura

研究成果: Article

6 被引用数 (Scopus)

抄録

Summary. To clarify the lineage involvement of haemopoietic progenitor cells in erythroleukaemia, the morphology and chromosomes of single colonies from a patient with erythroleukaemia were analysed simultaneously. The cytogenetic analysis of bone marrow cells revealed two clones; 44.XY, −7, −12, −17, del (5)(q31),+ Mar and 43,XY, −7, −12, −17, −19,del(5)(q31),+ Mar. Of 40 metaphases examined, there were 34 and six of these clones, respectively. Bone marrow mononuclear cells were plated at 5 × 104/ml in methylcellulose medium containing phytohaemagglutinin‐stimulated leucocyte conditioned medium and erythropoietin. Seventeen colonies, i.e. nine blast cell colonies, four myeloid (Sudan black B‐positive) colonies, and four erythroid (benzidine‐positive) colonies contained analysable metaphases, yielding 102 metaphases in total. Except for chromosome random loss, the karyotype within a colony remained constant. All three types of colonies showed an abnormal clone; 44.XY, − 7, −12, − 17,del(5)(q31),+Mar. From these findings, it is concluded that myeloid and erythroid lineages in erythroleukaemia were derived from the same abnormal clone.

本文言語English
ページ(範囲)647-656
ページ数10
ジャーナルBritish Journal of Haematology
64
4
DOI
出版ステータスPublished - 1986 12

ASJC Scopus subject areas

  • Hematology

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