Myoblast adhesion and proliferation on biodegradable polymer films with femtosecond laser-fabricated micro through-holes

Izumi Takayama, Naonari Kondo, Stefan Kalies, Alexander Heisterkamp, Mitsuhiro Terakawa

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Controlling cell adhesion and cell differentiation is necessary to fabricate a tissue with arbitrary properties for tissue engineering applications. A substrate with a porous structure as a cell scaffold allows the diffusion of the cell culture medium through the scaffold. In this work, we show that the femtosecond laser fabricated micro through-holes in biodegradable polymer films, enhance myoblast adhesion, and accelerates proliferation and differentiation. ChR2-C2C12 and UT-C2C12 cells were seeded on the films with micro through-holes each fabricated by a single femtosecond laser pulse. Cell adhesion was enhanced on films with holes fabricated by laser irradiation. In addition, cell proliferation was accelerated on films with micro through-holes that penetrate the film, compared to on films with micro craters that do not penetrate the film. On films with arrays consisting of micro through-holes, cells aligned along the arrays and cell fusion was enhanced, indicating the acceleration of cell differentiation.

本文言語English
論文番号e202000037
ジャーナルJournal of Biophotonics
13
7
DOI
出版ステータスPublished - 2020 7月 1

ASJC Scopus subject areas

  • 化学 (全般)
  • 材料科学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 工学(全般)
  • 物理学および天文学(全般)

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