Nano-scale physical surface coating of temperature-responsive polymers for cell sheet fabrication

Masamichi Nakayama, Yurika Kimura, Hideko Kanazawa, Masayuki Yamato, Teruo Okano

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

This study focused on the simple fabrication method of temperature-responsive cell culture surfaces by physical polymer coating. We synthesized diblock copolymers of poly(butyl methacrylate)-b-poly(N-isopropylacrylamide) (PBMA-b-PIPAAm) with various thermoresponsive PIPAAm chain length. Polymers were dissolved in mixed solvent of acetonitrile/N, N-dimethylformamide (5/1 in v/v), and the solution was deposited on the surface of commercial cell culture substrates using a spin coater. Chemically connected hydrophobic PBMA significantly enhanced physical absorption to target material surfaces and resulted in introducing thermoresponsive property to solid surfaces. To investigate temperature-dependent cellular behavior, bovine carotid artery endothelial cells were seeded on polymer-coated surfaces and cultured at 37 °C. Cells adhered and proliferated on temperature-responsive surface at 37 °C, while the efficiency of adhesion and proliferation became lower with increasing PIPAAm chain length. On the other hand, longer PIPAAm chains significantly promoted cell detachment from the surfaces by reducing temperature to 20 °C. Consequently, confluently cultured cells were successfully harvested as a single cell monolayer with maintaining cell-cell junctions and extracellular matrix proteins with simple low temperature treatment by choosing the adequate PIPAAm chain length of block copolymer.

本文言語English
ホスト出版物のタイトル2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014
出版社Institute of Electrical and Electronics Engineers Inc.
ISBN(電子版)9781479966790
DOI
出版ステータスPublished - 2015 1 9
イベント2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014 - Nagoya, Japan
継続期間: 2014 11 102014 11 12

出版物シリーズ

名前2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014

Other

Other2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014
国/地域Japan
CityNagoya
Period14/11/1014/11/12

ASJC Scopus subject areas

  • 電子工学および電気工学
  • 機械工学

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