Nanoimprinted holes to immobilize microbes

T. Kano, T. Inaba, N. Miki

研究成果: Conference contribution

抄録

In this paper we demonstrate highly dense immobilization of bacteria into nanoimprinted holes. Nanoimprinting enables micro holes smaller than 2 μm in diameter with a high accuracy, which cannot be patterned using conventional UV photolithography. In our prior work, we developed a microbial reactor immobilizing bacteria into micro holes, which facilitated collection and evaluation of reaction products while the number of bacteria involved in the reaction could be quantified. However, the holes were made by photolithography and the minimum size was limited to be 3 μm in diameter. Large holes allow multiple bacteria to be immobilized in a hole, which resulted in errors in quantification. The number of bacteria immobilized in a nanoimprinted hole was found to have smaller deviation than in photolithographically formed holes. In addition, density of the immobilized bacteria was experimentally found to be largest in case of 2-μm-holes. The proposed processes will be of great help for precise evaluation of bacteria reaction.

本文言語English
ホスト出版物のタイトルIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
ページ963-964
ページ数2
DOI
出版ステータスPublished - 2013 4 2
イベントIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
継続期間: 2013 1 202013 1 24

出版物シリーズ

名前Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(印刷版)1084-6999

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
国/地域Taiwan, Province of China
CityTaipei
Period13/1/2013/1/24

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 機械工学
  • 電子工学および電気工学

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