Nature of atrial natriuretic polypeptide in rat brain

Shozo Shiono, Kazuwa Nakao, Narito Morii, Takayuki Yamada, Hiroshi Itoh, Makoto Sakamoto, Akira Sugawara, Yoshihiko Saito, Goro Katsuura, Hiroo Imura

研究成果: Article査読

96 被引用数 (Scopus)

抄録

Using reverse phase high performance liquid chromatography (RP-HPLC) coupled with two radioimmunoassays for atrial natriuretic polypeptide (ANP) with different specificities, we investigated the nature of α-rat ANP-like immunoreactivity (α-rANP-LI) with a low molecular weight in the rat brain. Two major peaks with α-rANP-LI in the extract from rat whole brains were eluted in the vicinity of the elution position of synthetic α-rANP, a 28-amino acid polypeptide. These two components co-migrated with synthetic α-rANP(4-28) and α-rANP(5-28), respectively. The peak corresponding to α-rANP(4-28) was the highest, and only a little α-rANP-LI was detected at the elution position of α-rANP. An identical profile in RP-HPLC was also observed in the extract from the rat hypothalamus. These results indicate that the major components of α-rANP-LI with a low molecular weight in the rat brain are α-rANP(4-28) and α-rANP(5-28).

本文言語English
ページ(範囲)728-734
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
135
3
DOI
出版ステータスPublished - 1986 3月 28
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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