Near-infrared 1064 nm laser modulates migratory dendritic cells to augment the immune response to intradermal influenza vaccine

Kaitlyn Morse, Yoshifumi Kimizuka, Megan P.K. Chan, Mai Shibata, Yusuke Shimaoka, Shu Takeuchi, Benjamin Forbes, Christopher Nirschl, Binghao Li, Yang Zeng, Roderick T. Bronson, Wataru Katagiri, Ayako Shigeta, Ruxandra F. Srbulescu, Huabiao Chen, Rhea Y.Y. Tan, Kosuke Tsukada, Timothy Brauns, Jeffrey Gelfand, Ann SluderJoseph J. Locascio, Mark C. Poznansky, Niroshana Anandasabapathy, Satoshi Kashiwagi

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Brief exposure of skin to near-infrared (NIR) laser light has been shown to augment the immune response to intradermal vaccination and thus act as an immunologic adjuvant. Although evidence indicates that the NIR laser adjuvant has the capacity to activate innate subsets including dendritic cells (DCs) in skin as conventional adjuvants do, the precise immunological mechanism by which the NIR laser adjuvant acts is largely unknown. In this study we sought to identify the cellular target of the NIR laser adjuvant by using an established mouse model of intradermal influenza vaccination and examining the alteration of responses resulting from genetic ablation of specific DC populations. We found that a continuous wave (CW) NIR laser adjuvant broadly modulates migratory DC (migDC) populations, specifically increasing and activating the Lang+ and CD11b2Lang2 subsets in skin, and that the Ab responses augmented by the CW NIR laser are dependent on DC subsets expressing CCR2 and Langerin. In comparison, a pulsed wave NIR laser adjuvant showed limited effects on the migDC subsets. Our vaccination study demonstrated that the efficacy of the CW NIR laser is significantly better than that of the pulsed wave laser, indicating that the CW NIR laser offers a desirable immunostimulatory microenvironment for migDCs. These results demonstrate the unique ability of the NIR laser adjuvant to selectively target specific migDC populations in skin depending on its parameters, and highlight the importance of optimization of laser parameters for desirable immune protection induced by an NIR laser-adjuvanted vaccine.

本文言語English
ページ(範囲)1319-1332
ページ数14
ジャーナルJournal of Immunology
199
4
DOI
出版ステータスPublished - 2017 8月 15

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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