Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette

Masahiro Abe; Ryo Nishihara; Yuma Ikeda; Takahiro Nakajima; Moritoshi Sato; Naoko Iwasawa; Shigeru Nishiyama; Ramasamy Paulmurugan; Daniel Citterio; Sung Bae Kim; Koji Suzuki

doi:10.1002/cbic.201900149

Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette

Masahiro Abe, Ryo Nishihara, Yuma Ikeda, Takahiro Nakajima, Moritoshi Sato, Naoko Iwasawa, Shigeru Nishiyama, Ramasamy Paulmurugan, Daniel Citterio, Sung Bae Kim, Koji Suzuki

応用化学科

研究成果: Article

2 被引用数 (Scopus)

抄録

A coelenterazine (CTZ) analogue emitting near-infrared (NIR) bioluminescence was synthesized for through-bond energy transfer (TBET)-based imaging modalities. The analogue, named Cy5-CTZ, was prepared by conjugating cyanine-5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is intrinsically fluorescent and emits NIR-shifted luminescence upon reacting with an appropriate luciferase, the Renilla luciferase. This Cy5-CTZ substrate is optically stable in physiological samples and rapidly permeabilize through the plasma membrane into the cytosolic compartment of live cells.

本文言語	English
ページ（範囲）	1919-1923
ページ数	5
ジャーナル	ChemBioChem
巻	20
号	15
DOI	https://doi.org/10.1002/cbic.201900149
出版ステータス	Published - 2019

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

文献へのアクセス

10.1002/cbic.201900149

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引用スタイル

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abstract = "A coelenterazine (CTZ) analogue emitting near-infrared (NIR) bioluminescence was synthesized for through-bond energy transfer (TBET)-based imaging modalities. The analogue, named Cy5-CTZ, was prepared by conjugating cyanine-5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is intrinsically fluorescent and emits NIR-shifted luminescence upon reacting with an appropriate luciferase, the Renilla luciferase. This Cy5-CTZ substrate is optically stable in physiological samples and rapidly permeabilize through the plasma membrane into the cytosolic compartment of live cells.",

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author = "Masahiro Abe and Ryo Nishihara and Yuma Ikeda and Takahiro Nakajima and Moritoshi Sato and Naoko Iwasawa and Shigeru Nishiyama and Ramasamy Paulmurugan and Daniel Citterio and Kim, {Sung Bae} and Koji Suzuki",

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T1 - Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette

AU - Abe, Masahiro

AU - Nishihara, Ryo

AU - Ikeda, Yuma

AU - Nakajima, Takahiro

AU - Sato, Moritoshi

AU - Iwasawa, Naoko

AU - Nishiyama, Shigeru

AU - Paulmurugan, Ramasamy

AU - Citterio, Daniel

AU - Kim, Sung Bae

AU - Suzuki, Koji

PY - 2019

Y1 - 2019

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AB - A coelenterazine (CTZ) analogue emitting near-infrared (NIR) bioluminescence was synthesized for through-bond energy transfer (TBET)-based imaging modalities. The analogue, named Cy5-CTZ, was prepared by conjugating cyanine-5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is intrinsically fluorescent and emits NIR-shifted luminescence upon reacting with an appropriate luciferase, the Renilla luciferase. This Cy5-CTZ substrate is optically stable in physiological samples and rapidly permeabilize through the plasma membrane into the cytosolic compartment of live cells.

KW - bioluminescence

KW - dyes/pigments

KW - energy transfer

KW - fluorescence

KW - imaging agents

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