@article{787dcd41aa594389bed92ab61073eb38,
title = "Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette",
abstract = "A coelenterazine (CTZ) analogue emitting near-infrared (NIR) bioluminescence was synthesized for through-bond energy transfer (TBET)-based imaging modalities. The analogue, named Cy5-CTZ, was prepared by conjugating cyanine-5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is intrinsically fluorescent and emits NIR-shifted luminescence upon reacting with an appropriate luciferase, the Renilla luciferase. This Cy5-CTZ substrate is optically stable in physiological samples and rapidly permeabilize through the plasma membrane into the cytosolic compartment of live cells.",
keywords = "bioluminescence, dyes/pigments, energy transfer, fluorescence, imaging agents",
author = "Masahiro Abe and Ryo Nishihara and Yuma Ikeda and Takahiro Nakajima and Moritoshi Sato and Naoko Iwasawa and Shigeru Nishiyama and Ramasamy Paulmurugan and Daniel Citterio and Kim, {Sung Bae} and Koji Suzuki",
note = "Funding Information: This work was partly supported by JSPS KAKENHI grants no. 26288088, 15KK0029, 16K14051, 17H01215, and 24225001. Publisher Copyright: {\textcopyright} 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2019",
month = aug,
doi = "10.1002/cbic.201900149",
language = "English",
volume = "20",
pages = "1919--1923",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag",
number = "15",
}