Nedd9 protein, a Cas-L homologue, is upregulated after transient global ischemia in rats: Possible involvement of Nedd9 in the differentiation of neurons after ischemia

Takahiro Sasaki, Satoshi Iwata, Hirotaka James Okano, Yasuyo Urasaki, Junichi Hamada, Hirotoshi Tanaka, Nam H. Dang, Hideyuki Okano, Chikao Morimoto

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Background and Purpose - Some proteins involved in self-repair after stroke in the adult brain are primarily expressed during embryonic development and strongly down-regulated during the early postnatal phase. Neuronal precursor cell-expressed, developmentally down-regulated gene (Nedd) 9 was recognized to be identical to Crk-associated substrate lymphocyte type (Cas-L), a docking protein that associates with a variety of signaling molecules, such as focal adhesion kinase (FAK), proline-rich tyrosine kinase 2 (Pyk2), and Crk. We investigated the involvement of these proteins in the pathophysiology of global cerebral ischemia. Methods - The mouse Cas-L/Nedd9 cDNAs were cloned. The expression and function of Cas-L/Nedd9 protein in the pathogenesis of global ischemia in rats was investigated by RT-PCR, Western blot analysis, and immunohistochemistry. The neurite outgrowth of the transfectants of Nedd9 deletion mutants in PC-12 cells was also assessed to clarify the function of the Nedd9 protein. Results - Nedd9 was a splicing variant of Cas-L and was selectively induced in neurons of the cerebral cortex and hippocampus 1 to 14 days after the ischemia. Induced Nedd9 protein was tyrosine phosphorylated and was bound to FAK in dendrite and soma of neurons after the ischemia. Finally, it was demonstrated that Nedd9 promoted neurite outgrowth of PC-12 cells. Conclusions - Our study may support the potential of Nedd9 for participation in the differentiation of neurons after global ischemia in rats.

本文言語English
ページ(範囲)2457-2462
ページ数6
ジャーナルStroke
36
11
DOI
出版ステータスPublished - 2005 11

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialised Nursing

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