Neospora caninum: Tachyzoites express a potent type-I nucleoside triphosphate hydrolase, but lack nucleoside diphosphate hydrolase activity

Takashi Asai, Daniel K. Howe, Kyoko Nakajima, Tomoyoshi Nozaki, Tsutomu Takeuchi, L. David Sibley

研究成果: Article査読

43 被引用数 (Scopus)

抄録

We have identified type I nucleoside triphosphate hydrolase (NTPase; EC 3.6.1.3) activity, previously thought to be restricted to the virulent strains of Toxoplasma gondii, in the cell extracts of Neospora caninum tachyzoites. Sequence analysis of a complete cDNA from Nc-1 strain indicated that N. caninum NTPases shared approximately 69% identity to the NTPases of T. gondii and are most similar to the NTPase-I isozyme. Southern blot analysis of genomic DNA and sequence analysis of two independent NTP clones from the Nc-1 strain revealed the presence of multiple genes, at least two of which are transcribed. Substrate specificity and K(m) values for MgATP2 and MgADP- hydrolysis for recombinant or partially purified native NcNTPase were the same as those for the type I isozyme (NTPase-I). Significantly, no type II enzyme (NTPase-II) activity for NDP hydrolysis was detected in cell extracts of N. caninum, although it is universally present in all T. gondii strains that have been tested. This intriguing difference between these two closely related apicomplexan parasites may provide insight into the function of the NTPases during intracellular parasitism.

本文言語English
ページ(範囲)277-285
ページ数9
ジャーナルExperimental Parasitology
90
3
DOI
出版ステータスPublished - 1998 11月
外部発表はい

ASJC Scopus subject areas

  • 寄生虫科
  • 免疫学
  • 感染症

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