Nestin enhancer requirements for expression in normal and injured adult CNS

Clas B. Johansson, Carina Lothian, Magnus Molin, Hideyuki Okano, Urban Lendahl

研究成果: Article査読

45 被引用数 (Scopus)

抄録

The nestin gene is expressed in many CNS stem/progenitor cells, both in the embryo and the adult, and nestin is used commonly as a marker for these cells. In this report we analyze nestin enhancer requirements in the adult CNS, using transgenic mice carrying reporter genes linked to three different nestin enhancer constructs: the genomic rat nestin gene and 5 kb of upstream nestin sequence (NesP/acZ/3), 636 bp of the rat nestin second intron (E/nestin:EGFP), and a corresponding 714 bp region from the human second intron (Nes714tk/lacZ). NesP/acZ/3 and E/nestin:EGFP mice showed reporter gene expression in stem cell-containing regions of brain and spinal cord during normal conditions. NesP/acZ/3 and E/nestin:EGFP mice showed increased expression in spinal cord after injury and NesP/acZ/3 mice displayed elevated expression in the periventricular area of the brain after injury, which was not the case for the E/nestin:EGFP mice. In contrast, no expression in adult CNS in vivo was seen in the Nes714tk/lacZ mice carrying the human enhancer, neither during normal conditions nor after injury. The Nes714 tk/lacZ mice, however, expressed the reporter gene in reactive astrocytes and CNS stem cells cultured ex vivo. Collectively, this suggests a species difference for the nestin enhancer function in adult CNS and that elements outside the second intron enhancer are required for the full injury response in vivo.

本文言語English
ページ(範囲)784-794
ページ数11
ジャーナルJournal of neuroscience research
69
6
DOI
出版ステータスPublished - 2002 9月 15

ASJC Scopus subject areas

  • 細胞および分子神経科学

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