Network features and pathway analyses of a signal transduction cascade

Ryoji Yanashima, Noriyuki Kitagawa, Yoshiya Matsubara, Robert Weatheritt, Kotaro Oka, Shinichi Kikuchi, Masaru Tomita, Shun Ishizaki

研究成果: Article査読

6 被引用数 (Scopus)

抄録

The scale-free and small-world network models reflect the functional units of networks. However, when we investigated the network properties of a signaling pathway using these models, no significant differences were found between the original undirected graphs and the graphs in which inactive proteins were eliminated from the gene expression data. We analyzed signaling networks by focusing on those pathways that best reflected cellular function. Therefore, our analysis of pathways started from the ligands and progressed to transcription factors and cytoskeletal proteins. We employed the Python module to assess the target network. This involved comparing the original and restricted signaling cascades as a directed graph using microarray gene expression profi les of late onset Alzheimer's disease. The most commonly used method of shortest-path analysis neglects to consider the influences of alternative pathways that can affect the activation of transcription factors or cytoskeletal proteins. We therefore introduced included k-shortest paths and k-cycles in our network analysis using the Python modules, which allowed us to attain a reasonable computational time and identify k-shortest paths. This technique reflected results found in vivo and identified pathways not found when shortest path or degree analysis was applied. Our module enabled us to comprehensively analyse the characteristics of biomolecular networks and also enabled analysis of the effects of diseases considering the feedback loop and feedforward loop control structures as an alternative path.

本文言語English
論文番号13
ジャーナルFrontiers in Neuroinformatics
3
MAY
DOI
出版ステータスPublished - 2009 5 29

ASJC Scopus subject areas

  • 神経科学(その他)
  • 生体医工学
  • コンピュータ サイエンスの応用

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