Neurogenic potential of progenitors derived from human circulating CD14+ monocytes

Hiroaki Kodama, Takafumi Inoue, Ryuichi Watanabe, Daisuke Yasutomi, Yutaka Kawakami, Satoshi Ogawa, Katsuhiko Mikoshiba, Yasuo Ikeda, Masataka Kuwana

研究成果: Article査読

37 被引用数 (Scopus)

抄録

We previously reported a primitive cell fraction derived from human circulating CD14+ monocytes, named monocyte-derived multipotential cells (MOMC), that can differentiate along mesenchymal lineages, including bone, cartilage, fat, skeletal muscle and cardiac muscle. In this study, we investigated whether MOMC can differentiate into the neuronal lineage. MOMC were fluorescently labelled and cocultivated with a primary culture of rat neurons for up to 4 weeks. The protein and gene expressions of neuron-specific markers in the human MOMC were evaluated over time using immunohistochemistry, in situ hybridization and reverse transcription followed by PCR. Shortly after cocultivation with rat neurons, nearly all the MOMC expressed early neuroectodermal markers, Mash1, Neurogenin2 and NeuroD, together with nestin, an intermediate filament expressed in neurogenesis. After 14 days of coculture, a subpopulation of MOMC displayed a multipolar morphology with elongated neurites and expressed mature neuron-specific markers, including neurofilament, microtubule-associated protein type 2, β3-tubulin, NeuN and Hu. Transdifferentiation of monocytes into the neuroectodermal lineage was shown by the simultaneous expression of proneural markers and CD45/CD14 early in the differentiation process. The cocultivated MOMC retained their proliferative capacity for at least 16 days. Finally, the neuronal differentiation of MOMC was observed when they were cultured with neurons without cell-to-cell contact. The capacity of MOMC to differentiate into both mesodermal and neuroectodermal lineages suggests that circulating CD14+ monocytes are more multipotential than previously thought.

本文言語English
ページ(範囲)209-217
ページ数9
ジャーナルImmunology and Cell Biology
84
2
DOI
出版ステータスPublished - 2006 4 1

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学
  • 細胞生物学

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