Neuronal re-juvenilization in the nucleus ambiguus after vagal nerve injury

Takuji Takaoka, Akihiro Shiotani, Koichiro Saito, Masayuki Tomifuji, Yuko Mori, Takekatsu Fujimine, Hideyuki Okano, Kaoru Ogawa

研究成果: Article

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Nestin is an intermediate filament expressed in immature cells in the CNS including neural stem/progenitor cells, reactive astrocytes and immature neurons in lesser amounts after injury. Nestin expression in the nucleus ambiguus following vagal nerve injury was studied using nestin-EGFP transgenic rats. We confirmed that EGFP immunoreactivity was evident at 6 h to 8 days in ipsilateral nucleus ambiguus after nerve transection. Properties of these cells were examined immunohistochemically. These EGFP-immunoreactive cells were immunoreactive for Tuj1 and Hu, and exhibited ChAT activity. However, no immunoreactivity for GFAP or CNPase was observed. In normal development, the level of KCC2 expression is known to increase with maturation of neurons. In our study, decreased KCC2 expression was observed in nestin-EGFP-positive cells within the nucleus ambiguus on the lesioned side compared with the contralateral side. These EGFP-immunoreactive cells were immunonegative for BrdU. This is the first study to demonstrate the expression of a neural stem/progenitor cell-enriched marker, Nestin, in the nucleus ambiguus after vagal nerve injury. The present findings suggest that mature motoneurons are an origin of these Nestin-positive cells, which are induced after injury. Mature neurons in the nucleus ambiguus may thus have the potential to juvenilize after vagal/recurrent laryngeal nerve injury.

元の言語English
ページ(範囲)353-359
ページ数7
ジャーナルNeuroscience Research
65
発行部数4
DOI
出版物ステータスPublished - 2009 12 1

ASJC Scopus subject areas

  • Neuroscience(all)

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