Neuropoietin induces neuroepithelial cells to differentiate into astrocytes via activation of STAT3

Makoto Ohno, Jun Kohyama, Masakazu Namihira, Tsukasa Sanosaka, Jun A. Takahashi, Nobuo Hashimoto, Kinichi Nakashima

研究成果: Article

14 引用 (Scopus)

抄録

Neuropoietin (NP) is a recently identified member of the interleukin (IL)-6 family of cytokines, which share glycoprotein 130 (gp130) as a signal-transducing receptor component, and is highly expressed in embryonic brain. In this study, we show that NP has the potential to induce neuroepithelial cells to differentiate into astrocytes. NP stimulation leads to promoter activation of the gene for an astrocyte marker, glial fibrillary acidic protein (GFAP), which is clearly inhibited by either expression of a dominant-negative form of a transcription factor, signal transducer and activator of transcription 3 (STAT3) or by a nucleotide-substitution in the STAT3-binding element within the gene promoter. We further show that NP induces binding of endogenous STAT3 to its cognate sequence within the gfap gene promoter in neuroepithelial cells. Moreover, like the other IL-6 cytokine family members, NP promotes astrocyte differentiation in a synergistic manner with bone-morphogenetic protein (BMP)-2. Taken together, our data indicate that NP can be considered as a new astrocyte-inducing cytokine in the developing brain.

元の言語English
ページ(範囲)17-22
ページ数6
ジャーナルCytokine
36
発行部数1-2
DOI
出版物ステータスPublished - 2006 10
外部発表Yes

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Neuroepithelial Cells
STAT3 Transcription Factor
Astrocytes
Chemical activation
Genes
Cytokines
Interleukin-6
Brain
Bone Morphogenetic Protein 2
Glial Fibrillary Acidic Protein
Transcriptional Activation
Glycoproteins
Substitution reactions
Transcription Factors
Nucleotides

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

これを引用

Neuropoietin induces neuroepithelial cells to differentiate into astrocytes via activation of STAT3. / Ohno, Makoto; Kohyama, Jun; Namihira, Masakazu; Sanosaka, Tsukasa; Takahashi, Jun A.; Hashimoto, Nobuo; Nakashima, Kinichi.

:: Cytokine, 巻 36, 番号 1-2, 10.2006, p. 17-22.

研究成果: Article

Ohno, Makoto ; Kohyama, Jun ; Namihira, Masakazu ; Sanosaka, Tsukasa ; Takahashi, Jun A. ; Hashimoto, Nobuo ; Nakashima, Kinichi. / Neuropoietin induces neuroepithelial cells to differentiate into astrocytes via activation of STAT3. :: Cytokine. 2006 ; 巻 36, 番号 1-2. pp. 17-22.
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AU - Namihira, Masakazu

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AU - Takahashi, Jun A.

AU - Hashimoto, Nobuo

AU - Nakashima, Kinichi

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