New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

Toshio Morizane, Itsurou Satoh, Naoki Kumagai, Hidetsugu Saito, Akiko Iino, Yasutaka Inagaki, Tetsu Watanabe, Kanji Tsuchimoto, Masaharu Tsuchiya

研究成果: Article査読

2 被引用数 (Scopus)

抄録

A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.

本文言語English
ページ(範囲)225-229
ページ数5
ジャーナルJournal of Immunological Methods
91
2
DOI
出版ステータスPublished - 1986 7 24

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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