NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons

Ryu Yamanaka; Yutaka Shindo; Kohji Hotta; Koji Suzuki; Kotaro Oka

doi:10.1016/j.febslet.2013.06.049

NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoK_ATP channel opening in rat hippocampal neurons

Ryu Yamanaka, Yutaka Shindo, Kohji Hotta, Koji Suzuki, Kotaro Oka

研究成果: Article › 査読

20 被引用数 (Scopus)

抄録

Intracellular Mg²⁺ concentration ([_Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg²⁺ regulation, we visualized intracellular Mg ²⁺ using the fluorescent Mg²⁺ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [_Mg2+]i, and that Mg²⁺ mobilization is due to Mg²⁺ release from mitochondria induced by mitoK_ATP channel opening. In addition, Mg²⁺ release is potentiated by the positive feedback loop including mitoK_ATP channel opening, mitochondrial depolarization and PKC activation.

本文言語	English
ページ（範囲）	2643-2648
ページ数	6
ジャーナル	FEBS Letters
巻	587
号	16
DOI	https://doi.org/10.1016/j.febslet.2013.06.049
出版ステータス	Published - 2013 8 19

ASJC Scopus subject areas

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/j.febslet.2013.06.049

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引用スタイル

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title = "NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons",

abstract = "Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg 2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activation.",

keywords = "Hippocampal neuron, KMG-104 mitoK channel, Mg, Mitochondria",

author = "Ryu Yamanaka and Yutaka Shindo and Kohji Hotta and Koji Suzuki and Kotaro Oka",

note = "Funding Information: This work is supported by Grants-in-Aid for Scientific Research (A) ( 24240045 ), and Strategic Research Foundation Grant-aided Project for Private Universities from Ministry of Education , Culture, Sport, Science, and Technology, Japan (MEXT) , 2008–2012, ( S0801008 ).",

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AU - Yamanaka, Ryu

AU - Shindo, Yutaka

AU - Hotta, Kohji

AU - Suzuki, Koji

AU - Oka, Kotaro

N1 - Funding Information: This work is supported by Grants-in-Aid for Scientific Research (A) ( 24240045 ), and Strategic Research Foundation Grant-aided Project for Private Universities from Ministry of Education , Culture, Sport, Science, and Technology, Japan (MEXT) , 2008–2012, ( S0801008 ).

PY - 2013/8/19

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N2 - Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg 2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activation.

AB - Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg 2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activation.

KW - Hippocampal neuron

KW - KMG-104 mitoK channel

KW - Mg

KW - Mitochondria

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