Non-membranous granular organelle consisting of PCM-1: Subcellular distribution and cell-cycle-dependent assembly/disassembly

Akiharu Kubo, Shoichiro Tsukita

研究成果: Review article査読

89 被引用数 (Scopus)

抄録

Centriolar satellites were initially identified as electron-dense spherical granules, ∼70-100 nm in diameter, localized around the centrosomes. We have previously identified pericentriolar material 1 (PCM-1), with a molecular mass of ∼230 kDa, as a component of centriolar satellites. We now show by immunofluorescence microscopy that these granules are not only concentrated around centrioles but also scattered throughout the cytoplasm in various types of mouse cells, leading us tentatively to call them 'PCM-1 granules'. We then found that, when overexpressed, PCM-1 molecules lacking their C-terminal region bound directly with each other through two distinct regions to form large aggregates, which then recruited endogenous PCM-1. These large aggregates as well as endogenous PCM-1 granules appear to be disassembled during mitosis, and re-assembled when the cells entered interphase. These findings suggest that PCM-1 granules are formed by self-aggregation of PCM-1 and that this self-aggregation is regulated in a cell-cycle-dependent manner. Furthermore, we found that PCM-1 granules are distinct from pericentrin-containing granules, and that these two distinct types of granular structures are frequently associated with each other within the cytoplasm. These findings are discussed with special reference to the possible physiological functions of PCM-1 granules.

本文言語English
ページ(範囲)919-928
ページ数10
ジャーナルJournal of Cell Science
116
5
DOI
出版ステータスPublished - 2003 3 1
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

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