Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins

Yuta Abe, Koki Kamiya, Toshihisa Osaki, Hirotaka Sasaki, Ryuji Kawano, Norihisa Miki, Shoji Takeuchi

研究成果: Article査読

7 被引用数 (Scopus)

抄録

This paper describes a simple microfluidic device that can generate nonlinear concentration gradients. We changed the "width" of channels that can drastically shorten the total microfluidic channel length and simplify the microfluidic network design rather than the "length" of channels. The logarithmic concentration gradients generated by the device were in good agreement with those obtained by simulation. Using this device, we evaluated a probable IC50 value of the ABC transporter proteins by the competitive transport assays at five different logarithmic concentrations. This probable IC50 value was in good agreement with an IC50 value (0.92 μM) obtained at the diluted concentrations of seven points.

本文言語English
ページ(範囲)5557-5562
ページ数6
ジャーナルAnalyst
140
16
DOI
出版ステータスPublished - 2015 8 21

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 環境化学
  • 分光学
  • 電気化学

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