Novel conserved structural domains of acrosome reaction-inducing substance are widespread in invertebrates

Masahiro Naruse, Riho Ishikawa, Hiroshi Sakaya, Hideaki Moriyama, Motonori Hoshi, Midori Matsumoto

研究成果: Article査読

7 被引用数 (Scopus)

抄録

In the starfish Asterias amurensis, acrosome reaction inducing substance (ARIS) is the main factor responsible for allowing sperm to recognize the egg jelly and begin the acrosome reaction (AR). ARIS is a large proteoglycan-like molecule, and its pentasaccharide repeat, Fragment 1 (Fr. 1), is responsible for inducing AR. Here, we investigated the primary structure of ARIS for the first time in order to improve our understanding of its functionality. Electrophoretic analysis revealed that ARIS is a complex of three proteins, all of which are modified by the Fr. 1 sugar chain. Sequencing indicated that there are two novel, conserved domains in all three ARIS proteins: ARIS N-terminus (AR-N) and ARIS C-terminus (AR-C) domains. We also found that other echinoderms possess ARIS proteins that are capable of inducing the AR for homologous sperm, indicating that ARIS proteins may be a ubiquitous component for echinoderm fertilization. Moreover, we identified ARIS-like genes from Ctenophora to Protochordata.

本文言語English
ページ(範囲)57-66
ページ数10
ジャーナルMolecular reproduction and development
78
1
DOI
出版ステータスPublished - 2011 1 1

ASJC Scopus subject areas

  • 遺伝学
  • 発生生物学
  • 細胞生物学

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