Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms

Shigehiro Nagashima, Masayoshi Nakasako, Naoshi Dohmae, Masanari Tsujimura, Koji Takio, Masafumi Odaka, Masafumi Yohda, Nobuo Kamiya, Isao Endo

研究成果: Letter査読

326 被引用数 (Scopus)

抄録

The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme that is inactivated in the dark because of persistent association with NO and activated by photo-dissociation of NO. The crystal structure at 1.7 Å resolution and mass spectrometry revealed the structure of the non-heme iron catalytic center in the nitrosylated state. Two Cys residues coordinated to the iron were post-translationally modified to Cys-sulfenic and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO molecule. This unprecedented structure is likely to enable the photo-regulation of NHase and will provide an excellent model for designing photo-controllable chelate complexes and, ultimately, proteins.

本文言語English
ページ(範囲)347-351
ページ数5
ジャーナルNature Structural Biology
5
5
DOI
出版ステータスPublished - 1998 5月
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 生化学
  • 遺伝学

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