Novel non-thermal atrial fibrillation treatment with photosensitization reaction: Possibility of permanent electrical blockade in rat chronic model

Hiroki Matsuo, Arisa Ito, Shunichiro Miyoshi, Kyoko Soejima, Satoshi Ogawa, Tsunenori Arai

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

We demonstrated a possibility of electrical conduction block by ex vivo and in vivo experiments using rat models to establish a non-thermal treatment for atrial fibrillation by photosensitization reaction (PR). One hour after the injection of 2 mg/kg Talaporfin sodium to Wistar rat, the right ventricle (1.4 mmT) was extracted. Paced with a stimulation electrode, this tissue was placed in a tissue bath and immersed in irrigated Tyrode's solution of 37°C with 8 μg/ml Talaporfin sodium and the gas mixture bubbling of 95% CO2 and 5% O2. The propagated electrical signal was measured by two bipolar electrodes. Exciting light of 670 nm in wavelength was irradiated to the tissue between the bipolar electrodes by the power density of 1 W/cm 2. After this irradiation, propagation signal blockade was obtained and continued up to three hours. Rat atrioventricular (AV) node was employed as a target region for chronic model. The heart of Wistar rat was surgically exposed. External four-lead electrocardiogram of this rat was measured. Thirty minutes after the injection of 10 mg/kg Talaporfin sodium to the rat, exciting light of 663 nm in wavelength was irradiated to the AV node by the power density of 500 mW/cm2 for ten minutes. Acute AV block was obtained during the irradiation. Two weeks after this procedure, complete AV block was confirmed. The rat was sacrificed to obtain the tissue specimen. We found that the AV node was replaced by scarring tissue under the microscopic observation of the specimen. We verified possibility of permanent electrical conduction block using PR.

本文言語English
ホスト出版物のタイトル12th World Congress of the International Photodynamic Association - Photodynamic Therapy
ホスト出版物のサブタイトルBack to the Future
DOI
出版ステータスPublished - 2009 12月 1
イベント12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future - Seattle, WA, United States
継続期間: 2009 6月 112009 6月 15

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
7380
ISSN(印刷版)1605-7422

Other

Other12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future
国/地域United States
CitySeattle, WA
Period09/6/1109/6/15

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 原子分子物理学および光学
  • 放射線学、核医学およびイメージング

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