Novel PLA2G4C polymorphism as a molecular diagnostic assay for 19q loss in human gliomas

Christian Hartmann, Loki Johnk, Hikaru Sasaki, Robert B. Jenkins, David N. Louis

研究成果: Review article査読

4 被引用数 (Scopus)

抄録

PLA2G4C, encoding cytosolic phospholipase A2-γ (cPLA2-γ), is a 17-exon gene located on chromosome 19q13.3 within the putative glioma tumor suppressor gene region. Given the clinical importance of assessing 1p and 19q loss in human gliomas, the development of convenient and practical assays for detecting allelic loss is of considerable priority in neuro-oncology. We report a minisatellite polymorphism in the untranslated region of exon 1, with allelic variants that have one, two or three 27-bp repeats. The polymorphism is informative in 55.7% of a reference population, and accurately detects allelic loss of 19q in human gliomas. This novel marker offers distinct advantages for assessing 19q status in malignant gliomas. The relatively large size of the repeats allows detection of allelic variants with standard ethidium bromide-stained agarose gels and the PLA2G4C marker is the closest polymorphism to the smallest common deletion area in the putative glioma tumor suppressor gene region. These characteristics suggest that the PLA2G4C polymorphism will be a convenient and practical assay for clinical and research evaluation of 19q status in human gliomas.

本文言語English
ページ(範囲)178-182
ページ数5
ジャーナルBrain Pathology
12
2
DOI
出版ステータスPublished - 2002
外部発表はい

ASJC Scopus subject areas

  • 病理学および法医学
  • 神経科学(全般)
  • 臨床神経学

フィンガープリント

「Novel PLA2G4C polymorphism as a molecular diagnostic assay for 19q loss in human gliomas」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル