Nuclear magnetic resonance approaches for characterizing protein-protein interactions

Yuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa, Ichio Shimada

研究成果: Chapter

抄録

The gating of potassium ion (K+) channels is regulated by various kinds of protein-protein interactions (PPIs). Structural investigations of these PPIs provide useful information not only for understanding the gating mechanisms of K+ channels, but also for developing the pharmaceutical compounds targeting K+ channels. Here, we describe a nuclear magnetic resonance spectroscopic method, termed the cross saturation (CS) method, to accurately determine the binding surfaces of protein complexes, and its application to the investigation of the interaction between a G protein-coupled inwardly rectifying K+ channel and a G protein α subunit.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ115-128
ページ数14
DOI
出版ステータスPublished - 2018

出版物シリーズ

名前Methods in Molecular Biology
1684
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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