Nuclear transfer to study the nuclear reprogramming of human stem cells

Shigeo Saito, Ken Sawai, Yoshinobu Murayama, Keiichi Fukuda, Kazunari Yokoyama

研究成果: Chapter

抄録

Research of stem cells will enable us to understand the development and function of tissues and organs in mammals. The ability to induce regeneration of new tissues from embryonic stem (ES) cells derived from cloned blastocysts via nuclear transfer can be expected in the not-too-distant future. The fact that there is no way except nuclear cloning for the return of differentiated cells to undifferentiated cells remains an interesting problem to be solved. We describe protocols for the production of cloned calves from bovine ES cells to study nuclear reprogramming ability of stem cells. The frequency of term pregnancies for blastocysts from ES cells is higher than those of early pregnancies and maintained pregnancies after nuclear transfer with bovine somatic cells. We also describe protocols for gene introduction into bovine ES cells in vitro, particularly the human leukocyte antigens (HLA). Bovine ES cells provide a powerful tool for the generation of transgenic clonal offspring. This technique, when perfected for humans, may be critical for neural stem cell transplantation.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
ページ151-169
ページ数19
438
DOI
出版ステータスPublished - 2008
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
438
ISSN(印刷版)10643745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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