Nucleolar localization of DGCR8 and identification of eleven DGCR8-associated proteins

Aiko Shiohama, Takashi Sasaki, Setsuko Noda, Shinsei Minoshima, Nobuyoshi Shimizu

研究成果: Article査読

82 被引用数 (Scopus)

抄録

We identified 11 proteins that are associated with DGCR8 by immunoprecipitation assay and mass spectrometry. These proteins included Nucleolin, ILF3 and others, most of which appeared to be involved in the RNA processing or RNA transportation. We detected at least four kinds of protein complex, such as DROSHA/DGCR8, DGCR8/Nucleolin, DGCR8/ILF3 and ILF3/XPO5, by co-immunoprecipitation. The complex formation of DGCR8 with Nucleolin was dependent on RNA. Subcellular localization analysis by the immunofluorescent microscopy and immunoelectron microscopy indicated that DGCR8 locates at the nucleolus and small foci adjacent to splicing speckles in the nucleoplasm. Furthermore, the localization of DGCR8 at the nucleolus was changed by the inhibition of RNA transcription. Thus, our studies provided additional new evidence for the involvement of various protein complexes in the molecular mechanisms of apparently complex innate RNA interference machinery.

本文言語English
ページ(範囲)4196-4207
ページ数12
ジャーナルExperimental Cell Research
313
20
DOI
出版ステータスPublished - 2007 12 10

ASJC Scopus subject areas

  • 細胞生物学

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