Nucleophosmin as a candidate prognostic biomarker of ewing's sarcoma revealed by proteomics

Kazutaka Kikuta, Naobumi Tochigi, Tadakazu Shimoda, Hiroki Yabe, Hideo Morioka, Yoshiaki Toyama, Ako Hosono, Yasuo Eppu, Akira Kawai, Setsuo Hirohashi, Tadashi Kondo

研究成果: Article査読

43 被引用数 (Scopus)

抄録

Purpose: We aimed to identify novel prognostic biomarkers for Ewing's sarcoma by investigating the global protein expression profile of Ewing's sarcoma patients. Experimental Design: We examined the proteomic profile of eight biopsy samples from Ewing's sarcoma patients using two-dimensional difference gel electrophoresis. Three patients were alive and continuously disease-free over 3 years after the initial diagnosis (good prognosis group) and five had died of the disease within 2 years of the initial diagnosis (poor prognosis group). Results: The protein expression profiles produced using two-dimensional difference gel electrophoresis consisted of 2,364 protein spots, among which we identified 66 protein spots whose intensity showed >2-fold difference between the two patient groups. Mass spectrometric protein identification showed that the 66 spots corresponded to 53 distinct gene products. Pathway analysis revealed that 31 of 53 proteins, including nucleophosmin, were significantly related to bone tissue neoplasms (P < 0.000001). The prognostic performance of nucleophosmin was evaluated immunohistochemically on an additional 34 Ewing's sarcoma cases. Univariate and multivariate analyses revealed that nucleophosmin expression significantly correlated with overall survival (P < 0.01). Conclusions: These results establish nucleophosmin as a candidate of independent prognostic marker for Ewing's sarcoma patients. Measuring nucleophosmin in biopsy samples before treatment may contribute to the effective management of Ewing's sarcoma.

本文言語English
ページ(範囲)2885-2894
ページ数10
ジャーナルClinical Cancer Research
15
8
DOI
出版ステータスPublished - 2009 4月 15
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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