Numerical chromosome aberrations in hepatocellular carcinoma detected by fluorescence in situ hybridization

Naofumi Ohsawa, Michiie Sakamoto, Takao Saito, Michio Kobayashi, Setsuo Hirohashi

研究成果: Article

16 引用 (Scopus)

抄録

Aims/Methods: Numerical aberrations of chromosomes 7, 8, 9, 10, 12, 17, 18, X and Y were examined in 38 hepatocellular carcinoma specimens using centromere-specific DNA probes by the fluorescence in situ hybridization method. Results: Numerical aberrations in more than one of the chromosomes examined were found in 27 of 38 (71%) specimens; 6 of 15 (40%) well-differentiated hepatocellular carcinomas; 15 of 17 (88%) moderately differentiated hepatocellular carcinomas; and all of 6 (100%) poorly differentiated hepatocellular carcinomas, Of 6 early hepatocellular carcinomas, numerical chromosome aberrations were detected in 2. The incidence of numerical chromosome aberrations was 93.8% in patients with portal vein thromboses and/or intrahepatic metastases, 52.4% without portal vein thromboses and/or intrahepatic metastases (p < 0.05), while 89.5% of patients with a tumor more than 3 cm in diameter and 50.0% with a tumor less than 3 cm in diameter had chromosome aberrations (p < 0.05). Conclusions: These results suggest that numerical chromosome aberrations start to occur in the early stage of hepatocellular carcinoma and to accumulate during tumor progression.

元の言語English
ページ(範囲)655-662
ページ数8
ジャーナルJournal of Hepatology
25
発行部数5
DOI
出版物ステータスPublished - 1996 11
外部発表Yes

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Fluorescence In Situ Hybridization
Chromosome Aberrations
Hepatocellular Carcinoma
Portal Vein
Thrombosis
Neoplasm Metastasis
Chromosomes, Human, Pair 8
Neoplasms
Chromosomes, Human, Pair 7
Centromere
DNA Probes
Chromosomes
Incidence

ASJC Scopus subject areas

  • Gastroenterology

これを引用

Numerical chromosome aberrations in hepatocellular carcinoma detected by fluorescence in situ hybridization. / Ohsawa, Naofumi; Sakamoto, Michiie; Saito, Takao; Kobayashi, Michio; Hirohashi, Setsuo.

:: Journal of Hepatology, 巻 25, 番号 5, 11.1996, p. 655-662.

研究成果: Article

Ohsawa, Naofumi ; Sakamoto, Michiie ; Saito, Takao ; Kobayashi, Michio ; Hirohashi, Setsuo. / Numerical chromosome aberrations in hepatocellular carcinoma detected by fluorescence in situ hybridization. :: Journal of Hepatology. 1996 ; 巻 25, 番号 5. pp. 655-662.
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abstract = "Aims/Methods: Numerical aberrations of chromosomes 7, 8, 9, 10, 12, 17, 18, X and Y were examined in 38 hepatocellular carcinoma specimens using centromere-specific DNA probes by the fluorescence in situ hybridization method. Results: Numerical aberrations in more than one of the chromosomes examined were found in 27 of 38 (71{\%}) specimens; 6 of 15 (40{\%}) well-differentiated hepatocellular carcinomas; 15 of 17 (88{\%}) moderately differentiated hepatocellular carcinomas; and all of 6 (100{\%}) poorly differentiated hepatocellular carcinomas, Of 6 early hepatocellular carcinomas, numerical chromosome aberrations were detected in 2. The incidence of numerical chromosome aberrations was 93.8{\%} in patients with portal vein thromboses and/or intrahepatic metastases, 52.4{\%} without portal vein thromboses and/or intrahepatic metastases (p < 0.05), while 89.5{\%} of patients with a tumor more than 3 cm in diameter and 50.0{\%} with a tumor less than 3 cm in diameter had chromosome aberrations (p < 0.05). Conclusions: These results suggest that numerical chromosome aberrations start to occur in the early stage of hepatocellular carcinoma and to accumulate during tumor progression.",
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AU - Ohsawa, Naofumi

AU - Sakamoto, Michiie

AU - Saito, Takao

AU - Kobayashi, Michio

AU - Hirohashi, Setsuo

PY - 1996/11

Y1 - 1996/11

N2 - Aims/Methods: Numerical aberrations of chromosomes 7, 8, 9, 10, 12, 17, 18, X and Y were examined in 38 hepatocellular carcinoma specimens using centromere-specific DNA probes by the fluorescence in situ hybridization method. Results: Numerical aberrations in more than one of the chromosomes examined were found in 27 of 38 (71%) specimens; 6 of 15 (40%) well-differentiated hepatocellular carcinomas; 15 of 17 (88%) moderately differentiated hepatocellular carcinomas; and all of 6 (100%) poorly differentiated hepatocellular carcinomas, Of 6 early hepatocellular carcinomas, numerical chromosome aberrations were detected in 2. The incidence of numerical chromosome aberrations was 93.8% in patients with portal vein thromboses and/or intrahepatic metastases, 52.4% without portal vein thromboses and/or intrahepatic metastases (p < 0.05), while 89.5% of patients with a tumor more than 3 cm in diameter and 50.0% with a tumor less than 3 cm in diameter had chromosome aberrations (p < 0.05). Conclusions: These results suggest that numerical chromosome aberrations start to occur in the early stage of hepatocellular carcinoma and to accumulate during tumor progression.

AB - Aims/Methods: Numerical aberrations of chromosomes 7, 8, 9, 10, 12, 17, 18, X and Y were examined in 38 hepatocellular carcinoma specimens using centromere-specific DNA probes by the fluorescence in situ hybridization method. Results: Numerical aberrations in more than one of the chromosomes examined were found in 27 of 38 (71%) specimens; 6 of 15 (40%) well-differentiated hepatocellular carcinomas; 15 of 17 (88%) moderately differentiated hepatocellular carcinomas; and all of 6 (100%) poorly differentiated hepatocellular carcinomas, Of 6 early hepatocellular carcinomas, numerical chromosome aberrations were detected in 2. The incidence of numerical chromosome aberrations was 93.8% in patients with portal vein thromboses and/or intrahepatic metastases, 52.4% without portal vein thromboses and/or intrahepatic metastases (p < 0.05), while 89.5% of patients with a tumor more than 3 cm in diameter and 50.0% with a tumor less than 3 cm in diameter had chromosome aberrations (p < 0.05). Conclusions: These results suggest that numerical chromosome aberrations start to occur in the early stage of hepatocellular carcinoma and to accumulate during tumor progression.

KW - Centromere probe

KW - Chromosome abnormalities

KW - Polysomy

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