Occurrence of a novel cardiac natriuretic peptide in rats

Hiroshi Itoh, Kazuwa Nakao, Yoshikazu Kambayashi, Kiminori Hosoda, Yoshihiko Saito, Takayuki Yamada, Masashi Mukoyama, Hiroshi Arai, Gotaro Shirakami, Shin ichi Suga, Ikuyo Yoshida, Ken Inouye, Hiroo Imura

研究成果: Article査読

42 被引用数 (Scopus)

抄録

We established a specific radioimmunoassay for the ring structure of "iso-ANP" and detected iso-ANP[23-46]-like immunoreactivity (-LI) in the rat atrium (2.76 ± 0.5 μg/g) and ventricle (13.9 ± 5.7 ng/g). High performance-gel permeation chromatography revealed that iso-ANP[23-46]-LI in the rat heart was composed of two components with molecular weights of 10K and 5K. In reverse phase-high performance liquid chromatography, the retention times of these components were clearly different from that of synthetic iso-ANP. The 5K peptide was demonstrated to be present in the perfusate from isolated rat hearts and possessed binding ability to ANP receptors. This natriuretic peptide was, however, not detectable in other tissues including the brain. We conclude that the novel cardiac natriuretic peptide distinct from iso-ANP and ANP occurs in the rat heart and is secreted from the heart.

本文言語English
ページ(範囲)732-739
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
161
2
DOI
出版ステータスPublished - 1989 6月 15
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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