TY - JOUR
T1 - Ocular-component-specific miRNA expression in a murine model of lens-induced myopia
AU - Tanaka, Yasuhisa
AU - Kurihara, Toshihide
AU - Hagiwara, Yumi
AU - Ikeda, Shin Ichi
AU - Mori, Kiwako
AU - Jiang, Xiaoyan
AU - Torii, Hidemasa
AU - Tsubota, Kazuo
N1 - Funding Information:
Funding: This work was supported by Santen Pharmaceutical Co., LTD (Osaka, Japan), and was a collaborative study between Keio University and Santen.
Funding Information:
This work was supported by Santen Pharmaceutical Co., LTD (Osaka, Japan), and was a collaborative study between Keio University and Santen. We would like to thank Keiko Takahashi, a member of the Laboratory of Photobiology, Keio University School of Medicine and Masatsugu Nakamura, Masatomo Kato, Koushi Fujisawa, and Kenji Imoto of Santen Pharmaceutical Co., LTD (Osaka, Japan) for their technical and administrative support.
Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - To identify tissues and molecules involved in refractive myopic shift and axial length elongation in a murine lens-induced myopia model, we performed a comprehensive analysis of microRNA (miRNA) expression. Three weeks after negative 30 diopter lens fixation on three-week-old C57BL/6J mice, total RNA was extracted from individual ocular components including cornea, iris, lens, retina, retinal pigment epithelium (RPE)/choroid, and sclera tissue. The miRNA expression analysis was pooled from three samples and carried out using Agilent Mouse miRNA Microarray (8 × 60 K) miRBase21.0. The expression ratio was calculated, and differentially expressed miRNAs were extracted, using GeneSpring GX 14.5. Myopic induction showed a significant myopic refractive change, axial elongation, and choroidal thinning. Through the comprehensive miRNA analysis, several upregulated miRNAs (56 in cornea tissue, 13 in iris tissue, 6 in lens tissue, 0 in retina tissue, 29 in RPE/choroid tissue, and 30 in sclera tissue) and downregulated miRNAs (7 in cornea tissue, 28 in iris tissue, 17 in lens tissue, 9 in retina tissue, 7 in RPE/choroid tissue, and 40 in sclera tissue) were observed. Overlapping expression changes in miRNAs were also found in different ocular components. Some of this miRNA dysregulation may be functionally involved in refractive myopia shift and axial length elongation.
AB - To identify tissues and molecules involved in refractive myopic shift and axial length elongation in a murine lens-induced myopia model, we performed a comprehensive analysis of microRNA (miRNA) expression. Three weeks after negative 30 diopter lens fixation on three-week-old C57BL/6J mice, total RNA was extracted from individual ocular components including cornea, iris, lens, retina, retinal pigment epithelium (RPE)/choroid, and sclera tissue. The miRNA expression analysis was pooled from three samples and carried out using Agilent Mouse miRNA Microarray (8 × 60 K) miRBase21.0. The expression ratio was calculated, and differentially expressed miRNAs were extracted, using GeneSpring GX 14.5. Myopic induction showed a significant myopic refractive change, axial elongation, and choroidal thinning. Through the comprehensive miRNA analysis, several upregulated miRNAs (56 in cornea tissue, 13 in iris tissue, 6 in lens tissue, 0 in retina tissue, 29 in RPE/choroid tissue, and 30 in sclera tissue) and downregulated miRNAs (7 in cornea tissue, 28 in iris tissue, 17 in lens tissue, 9 in retina tissue, 7 in RPE/choroid tissue, and 40 in sclera tissue) were observed. Overlapping expression changes in miRNAs were also found in different ocular components. Some of this miRNA dysregulation may be functionally involved in refractive myopia shift and axial length elongation.
KW - Lens-induced myopia
KW - Ocular components
KW - miRNA
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U2 - 10.3390/ijms20153629
DO - 10.3390/ijms20153629
M3 - Article
C2 - 31344984
AN - SCOPUS:85070615995
VL - 20
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 15
M1 - 3629
ER -