Optical bioimaging: From living tissue to a single molecule: Imaging and functional analysis of blood flow in organic microdilution

Haruyuki Minamitani, Kosuke Tsukada, Eiichi Sekizuka, Chikara Oshio

研究成果: Article査読

19 被引用数 (Scopus)

抄録

Activity of blood cells, erythrocytes, leucocytes, and platelets, in microcirculation was observed by using an intravital microscope and confocal laser scanning microscope connected with an image processing system including fluorescence and phosphorescence emission methods. Dynamic functions of the blood flow were mainly observed in mesentery, brain, and liver tissues of rats. The results are summarized as follows: Deformability of diabetic erythrocytes was significantly lower than that of healthy controls, particularly at high shear rate. The spring constant and Young's modulus of diabetic erythrocytes obviously stiffened, making them hard to deform in the capillary. During hemorrhagic shock and thrombosis, flow velocity and oxygen partial pressure of blood decreased in the brain and liver tissues that can be visualized by using FITC stained erythrocytes and Pd-porphyrin derivative as a pO2 probe. Platelet adhesion and thrombus formation in the micro-vessels accelerated under the photodynamic reaction; diabetic platelets showed augmented adhesion and aggregation on the vessel wall which was followed by acute thromboembolism. Active oxygen radicals take part in thrombus formation, accompanied with adhesion of the activated leucocytes. Fluorescent dye probes, rhodamine G and acridine orange, are quite useful for visualization of the flow behavior of platelets and leucocytes, respectively.

本文言語English
ページ(範囲)227-233
ページ数7
ジャーナルJournal of Pharmacological Sciences
93
3
DOI
出版ステータスPublished - 2003 11月
外部発表はい

ASJC Scopus subject areas

  • 分子医療
  • 薬理学

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