Optical detection of DNA translocation through silicon nanopore by ultraviolet light

Hirohito Yamazaki, Shinji Kimura, Mutsumi Tsukahara, Keiko Esashika, Toshiharu Saiki

研究成果: Article査読

16 被引用数 (Scopus)

抄録

In this paper, we propose a new optical detection scheme for nanopore-based DNA sequencing with high resolution towards eventual base identification. We use ultraviolet light for excitation of a fluorescent probe attached to DNA and a nanopore in the silicon membrane that has a significantly large refractive index and an extinction coefficient at ultraviolet wavelengths. In this study, numerical electromagnetic simulation revealed that the z-polarization component (perpendicular to the membrane plane) of the electric field was dominant near the nanopore and generated a large electric field gradient at the nanopore exit, typically with a decay length of 2 nm for a nanopore with diameter of 7 nm. The large extinction coefficient contributed to reduction in background noise coming from fluorophore-labeled DNA strands that remain behind the membrane (the cis side of the membrane). We observed a high signal-to-noise ratio of single DNA translocation events under the application of an electric field.

本文言語English
ページ(範囲)53-56
ページ数4
ジャーナルApplied Physics A: Materials Science and Processing
115
1
DOI
出版ステータスPublished - 2014 4

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)

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