Optical monitoring of the neural activity evoked by mechanical stimulation in the earthworm nervous system with a fluorescent dye, FM1-43

Ryosaku Shimizu, Kotaro Oka, Hiroto Ogawa, Keisuke Suzuki, Junichi Saito, Kenji Mizutani, Kazuo Tanishita

研究成果: Article査読

11 被引用数 (Scopus)

抄録

In the central nervous system of the earthworm, sensory and motor neurons have direct synapses on three giant fibers. To determine the locations of synapses and neural network activated by mechanical stimuli, we optically monitored the activity-dependent staining in the earthworm ventral nerve cord with a styryl dye, N-(3-triethylammoniumpropyl)-4- (4(dibutylamino)styryl)pyridinium dibromide (FM1-43), and a confocal laser scanning microscope. When scratch stimulus was applied to the body wall of the earthworm, bright fluorescent spots with 3-10 μm in diameter localized only in the stimulated segmental ganglion of the ventral nerve cord. The fluorescent intensity of these spots decreased during dye-free high K+ saline incubation. These results suggest that FM1-43 is useful for activity- dependent staining of invertebrate neurons and their synaptic regions as well as vertebrate nervous system.

本文言語English
ページ(範囲)159-162
ページ数4
ジャーナルNeuroscience Letters
268
3
DOI
出版ステータスPublished - 1999 6 25

ASJC Scopus subject areas

  • 神経科学(全般)

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