Optical observation of DNA motion during and immediately after nanopore translocation

Hirohito Yamazaki, Shintaro Ito, Keiko Esashika, Toshiharu Saiki

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Herein, we report an optical nanopore detection system capable of investigating DNA motion near nanopores not only during translocation but also post-translocation with submillisecond resolution. Using our optical nanopore detection system, we observed the voltage dependence of the dwell time of both 10-kbp double-stranded DNA (dsDNA) and lambda DNA in the excitation volume, which can be attributed to the drift-dominated motion. We found that the lambda DNA had slower drift motion than 10-kbp dsDNA, indicating that DNA with longer gyration experiences a lower nonuniform electric force.

本文言語English
論文番号017001
ジャーナルApplied Physics Express
9
1
DOI
出版ステータスPublished - 2016 1

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)

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