Optical observation of DNA motion during and immediately after nanopore translocation

Hirohito Yamazaki, Shintaro Ito, Keiko Esashika, Toshiharu Saiki

研究成果: Article

6 引用 (Scopus)

抄録

Herein, we report an optical nanopore detection system capable of investigating DNA motion near nanopores not only during translocation but also post-translocation with submillisecond resolution. Using our optical nanopore detection system, we observed the voltage dependence of the dwell time of both 10-kbp double-stranded DNA (dsDNA) and lambda DNA in the excitation volume, which can be attributed to the drift-dominated motion. We found that the lambda DNA had slower drift motion than 10-kbp dsDNA, indicating that DNA with longer gyration experiences a lower nonuniform electric force.

元の言語English
記事番号017001
ジャーナルApplied Physics Express
9
発行部数1
DOI
出版物ステータスPublished - 2016 1 1

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Nanopores
dwell
gyration
DNA
deoxyribonucleic acid
Electric potential
electric potential
excitation

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

これを引用

Optical observation of DNA motion during and immediately after nanopore translocation. / Yamazaki, Hirohito; Ito, Shintaro; Esashika, Keiko; Saiki, Toshiharu.

:: Applied Physics Express, 巻 9, 番号 1, 017001, 01.01.2016.

研究成果: Article

Yamazaki, Hirohito ; Ito, Shintaro ; Esashika, Keiko ; Saiki, Toshiharu. / Optical observation of DNA motion during and immediately after nanopore translocation. :: Applied Physics Express. 2016 ; 巻 9, 番号 1.
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